Frontiers in plant science 2016
OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance in Oryza sativa.
Peroxisomes are single membrane-bound organelles, whose basic enzymatic constituents are catalase and H2O2-producing flavin oxidases. Previous reports showed that peroxisome is involved in numerous processes including primary and secondary metabolism, plant development and abiotic stress responses. However, knowledge on the function of different peroxisome genes from rice and its regulatory roles in salt and other abiotic stresses is limited. Here, a novel prey protein, OsPEX11 (Os03g0302000), was screened and identified by yeast two-hybrid and GST pull-down assays. Phenotypic analysis of OsPEX11 overexpression seedlings demonstrated that they had better tolerance to salt stress than wild type (WT) and OsPEX11-RNAi seedlings. Compared with WT and OsPEX11-RNAi seedlings, overexpression of OsPEX11 had lower level of lipid peroxidation, Na(+)/K(+) ratio, higher activities of antioxidant enzymes (SOD, POD, and CAT) and proline accumulation. Furthermore, qPCR data suggested that OsPEX11 acted as a positive regulator of salt tolerance by reinforcing the rm' data-tid='220' href='#term-220'>='term' data-tid='219' href='#term-219'>8' href='#term-218'>='term' data-tid='217' href='#term-217'>term' data-tid='216' href='#term-216'>expression of several well-known rice transporters (OsHKT2;1, OsHKT1;5, OsLti6a, OsLti6b, OsSOS1, OsNHX1, and OsAKT1) involved in Na(+)/K(+) homeostasis in transgenic plants under salinity. Ultrastructural observations of OsPEX11-RNAi seedlings showed that they were less sensitive to salt stress than WT and overexpression lines. These results provide experimental evidence that OsPEX11 is an important gene implicated in Na(+) and K(+) regulation, and plays a critical role in salt stress tolerance by modulating the expression of cation transporters and antioxidant defense. Thus, OsPEX11 could be considered in transgenic breeding for improvement of salt stress tolerance in rice crop.
|salt tolerance||Os01g0348900||Q0JMY8 B7E7K3|
|expressi||Os06g0701700||Q0D9S3 A0A0P0X0T3 A0A0N7KMP0|
|term'||Os06g0701700||Q0D9S3 A0A0P0X0T3 A0A0N7KMP0|
|<a class||Os01g0307500||Q0JNB6 B9EVS1|
|8' hre||Os12g0641100||Q0ILJ6 Q5ICN3 A0A0P0YDP3 A0A0P0YCQ9|
|rm' da||Os01g0648000||Q0JKV1 A0A0P0V5X9|